› 4lhl

X-ray diffraction
1.43Å resolution

Structure of the N-terminal domain of the Flo1 adhesin (N-Flo1p) from the yeast Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb4lhl/pdb
PDB entry 4lhl coloured by chain, front view.
PDB entry 4lhl coloured by chain, side view.
PDB entry 4lhl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival.
Goossens KV, Ielasi FS, Nookaew I, Stals I, Alonso-Sarduy L, Daenen L, Van Mulders SE, Stassen C, van Eijsden RG, Siewers V, Delvaux FR, Kasas S, Nielsen J, Devreese B, Willaert RG
mBio 6 (2015)
PMID: 25873380

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flocculation protein FLO1 Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32768 (Residues: 23-271; Coverage: 16%)
Gene names: FLO1, FLO2, FLO4, FLO8, YAR050W
Sequence domains: PA14 domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 45.8Å b: 61.77Å c: 106.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.165
Expression system: Escherichia coli

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Citations

9 review citations

Amyloid-Like β-Aggregates as Force-Sensitive Switches in Fungal Biofilms and Infections.
Lipke et al. (2018)
8 more

3 mentions without citation

Adhesins of Yeasts: Protein Structure and Interactions.
Willaert RG. (2018)
2 more