PDBe 4hwr

X-ray diffraction
1.9Å resolution

Crystal structure of E. coli Threonyl-tRNA synthetase bound to a novel inhibitor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A8M3 (Residues: 242-642; Coverage: 63%)
Gene names: JW1709, b1719, thrS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 156.899Å b: 109.777Å c: 86.995Å
α: 90° β: 123.76° γ: 90°
R-values:
R R work R free
0.267 0.266 0.303
Expression system: Escherichia coli BL21