4e6h › Citations

Crystal structure of the Rna14-Rna15 complex.

Paulson AR, Tong L
RNA 18 1154-62 (2012)
Related entries: 4e85, 4eba

Cited: 16 times
EuropePMC logo PMID: 22513198

Abstract

A large protein machinery is required for 3'-end processing of mRNA precursors in eukaryotes. Cleavage factor IA (CF IA), a complex in the 3'-end processing machinery in yeast, contains four subunits, Rna14, Rna15, Clp1, and Pcf11. Rna14 has a HAT (half a TPR) domain at the N terminus and a region at the C terminus that mediates interactions with Rna15. Rna15 contains a RNA recognition module (RRM) at the N terminus, followed by a hinge region. These two proteins are homologs of CstF-77 and CstF-64 in the cleavage stimulation factor (CstF) of the mammalian 3'-end processing machinery. We report the first crystal structure of Rna14 in complex with the hinge region of Rna15, and the structures of the HAT domain of Rna14 alone in two different crystal forms. The complex of the C-terminal region of Rna14 with the hinge region of Rna15 does not have strong interactions with the HAT domain of Rna14, and this complex is likely to function independently of the HAT domain. Like CstF-77, the HAT domain of Rna14 is also a tightly associated dimer with a highly elongated shape. However, there are large variations in the organization of this dimer among the Rna14 structures, and there are also significant structural differences to CstF-77. These observations suggest that the HAT domain and especially its dimer may have some inherent conformational variability.

Reviews - 4e6h mentioned but not cited (1)

  1. Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery. Xiang K, Tong L, Manley JL. Mol Cell Biol 34 1894-1910 (2014)

Articles - 4e6h mentioned but not cited (2)

  1. Structure and function of pre-mRNA 5'-end capping quality control and 3'-end processing. Jurado AR, Tan D, Jiao X, Kiledjian M, Tong L. Biochemistry 53 1882-1898 (2014)
  2. Defining the Domain Arrangement of the Mammalian Target of Rapamycin Complex Component Rictor Protein. Zhou P, Zhang N, Nussinov R, Ma B. J Comput Biol 22 876-886 (2015)


Reviews citing this publication (2)

  1. Recent molecular insights into canonical pre-mRNA 3'-end processing. Sun Y, Hamilton K, Tong L. Transcription 11 83-96 (2020)
  2. Molecular Insights into mRNA Polyadenylation and Deadenylation. Liu J, Lu X, Zhang S, Yuan L, Sun Y. Int J Mol Sci 23 10985 (2022)

Articles citing this publication (11)

  1. Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex. Hill CH, Boreikaitė V, Kumar A, Casañal A, Kubík P, Degliesposti G, Maslen S, Mariani A, von Loeffelholz O, Girbig M, Skehel M, Passmore LA. Mol Cell 73 1217-1231.e11 (2019)
  2. Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery. Zhang Y, Sun Y, Shi Y, Walz T, Tong L. Mol Cell 77 800-809.e6 (2020)
  3. Reconstitution of the CstF complex unveils a regulatory role for CstF-50 in recognition of 3'-end processing signals. Yang W, Hsu PL, Yang F, Song JE, Varani G. Nucleic Acids Res 46 493-503 (2018)
  4. Structural basis for recruiting and shuttling of the spliceosomal deubiquitinase USP4 by SART3. Park JK, Das T, Song EJ, Kim EE. Nucleic Acids Res 44 5424-5437 (2016)
  5. PALE CRESS binds to plastid RNAs and facilitates the biogenesis of the 50S ribosomal subunit. Meurer J, Schmid LM, Stoppel R, Leister D, Brachmann A, Manavski N. Plant J 92 400-413 (2017)
  6. Structural Basis of the Recruitment of Ubiquitin-specific Protease USP15 by Spliceosome Recycling Factor SART3. Zhang Q, Harding R, Hou F, Dong A, Walker JR, Bteich J, Tong Y. J Biol Chem 291 17283-17292 (2016)
  7. Structural and biochemical analysis of the assembly and function of the yeast pre-mRNA 3' end processing complex CF I. Barnwal RP, Lee SD, Moore C, Varani G. Proc Natl Acad Sci U S A 109 21342-21347 (2012)
  8. The C terminus of Pcf11 forms a novel zinc-finger structure that plays an essential role in mRNA 3'-end processing. Yang F, Hsu P, Lee SD, Yang W, Hoskinson D, Xu W, Moore C, Varani G. RNA 23 98-107 (2017)
  9. Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing. Guéguéniat J, Dupin AF, Stojko J, Beaurepaire L, Cianférani S, Mackereth CD, Minvielle-Sébastia L, Fribourg S. Nucleic Acids Res 45 10115-10131 (2017)
  10. Molecular mechanism for the interaction between human CPSF30 and hFip1. Hamilton K, Tong L. Genes Dev 34 1753-1761 (2020)
  11. Increased versatility despite reduced molecular complexity: evolution, structure and function of metazoan splicing factor PRPF39. De Bortoli F, Neumann A, Kotte A, Timmermann B, Schüler T, Wahl MC, Loll B, Heyd F. Nucleic Acids Res 47 5867-5879 (2019)


Quick links