› 4pz1

X-ray diffraction
1.73Å resolution

Crystal structure of a sHIP (UniProt Id: Q99XU0) mutant from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb4pz1/pdb
PDB entry 4pz1 coloured by chain, front view.
PDB entry 4pz1 coloured by chain, side view.
PDB entry 4pz1 coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Functional and structural properties of a novel protein and virulence factor (Protein sHIP) in Streptococcus pyogenes.
Wisniewska M, Happonen L, Kahn F, Varjosalo M, Malmström L, Rosenberger G, Karlsson C, Cazzamali G, Pozdnyakova I, Frick IM, Björck L, Streicher W, Malmström J, Wikström M
J Biol Chem 289 18175-88 (2014)
PMID: 24825900

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF4298 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.35 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99XU0 (Residues: 3-98; Coverage: 98%)
Gene name: SPy_2034
Sequence domains: Domain of unknown function (DUF4298)

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Unit cell:
a: 29.37Å b: 33.28Å c: 89.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.211
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Functional Regulation of the Plasma Protein Histidine-Rich Glycoprotein by Zn2+ in Settings of Tissue Injury.
Priebatsch et al. (2017)
3 other articles

1 mention without citation

Structure and Interactions of a Dimeric Variant of sHIP, a Novel Virulence Determinant of Streptococcus pyogenes.
Diehl et al. (2016)