› 3tjo

X-ray diffraction
2.3Å resolution

HtrA1 catalytic domain, mutationally inactivated

Released:
DOI: 10.2210/pdb3tjo/pdb
PDB entry 3tjo coloured by chain, front view.
PDB entry 3tjo coloured by chain, side view.
PDB entry 3tjo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of HtrA1 and its subdomains.
Eigenbrot C, Ultsch M, Lipari MT, Moran P, Lin SJ, Ganesan R, Quan C, Tom J, Sandoval W, van Lookeren Campagne M, Kirchhofer D
Structure 20 1040-50 (2012)
PMID: 22578544

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease HTRA1 Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 231 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92743 (Residues: 161-370; Coverage: 46%)
Gene names: HTRA, HTRA1, PRSS11
Sequence domains: Trypsin-like peptidase domain
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand BOG 2 x BOG
Ligand SO4 4 x SO4
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 151.922Å b: 151.922Å c: 87.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.216
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Features of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy.
Nozaki et al. (2014)
8 more

4 mentions without citation

Structural and Functional Analysis of Human HtrA3 Protease and Its Subdomains.
Glaza et al. (2015)
3 more