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Structural basis for activation of class Ib ribonucleotide reductase.

Boal AK, Cotruvo JA, Stubbe J, Rosenzweig AC
Science 329 1526-30 (2010)
Related entries: 3n37, 3n38, 3n39, 3n3b

Cited: 83 times
EuropePMC logo PMID: 20688982

Abstract

The class Ib ribonucleotide reductase of Escherichia coli can initiate reduction of nucleotides to deoxynucleotides with either a Mn(III)2-tyrosyl radical (Y•) or a Fe(III)2-Y• cofactor in the NrdF subunit. Whereas Fe(III)2-Y• can self-assemble from Fe(II)2-NrdF and O2, activation of Mn(II)2-NrdF requires a reduced flavoprotein, NrdI, proposed to form the oxidant for cofactor assembly by reduction of O2. The crystal structures reported here of E. coli Mn(II)2-NrdF and Fe(II)2-NrdF reveal different coordination environments, suggesting distinct initial binding sites for the oxidants during cofactor activation. In the structures of Mn(II)2-NrdF in complex with reduced and oxidized NrdI, a continuous channel connects the NrdI flavin cofactor to the NrdF Mn(II)2 active site. Crystallographic detection of a putative peroxide in this channel supports the proposed mechanism of Mn(III)2-Y• cofactor assembly.

Reviews - 3n3a mentioned but not cited (3)

  1. Metallation and mismetallation of iron and manganese proteins in vitro and in vivo: the class I ribonucleotide reductases as a case study. Cotruvo JA, Stubbe J. Metallomics 4 1020-1036 (2012)
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Articles - 3n3a mentioned but not cited (5)

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  4. Redox-induced structural changes in the di-iron and di-manganese forms of Bacillus anthracis ribonucleotide reductase subunit NrdF suggest a mechanism for gating of radical access. Grāve K, Lambert W, Berggren G, Griese JJ, Bennett MD, Logan DT, Högbom M. J Biol Inorg Chem 24 849-861 (2019)
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