› 2yfr

X-ray diffraction
1.75Å resolution

Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533

Released:
DOI: 10.2210/pdb2yfr/pdb
PDB entry 2yfr coloured by chain, front view.
PDB entry 2yfr coloured by chain, side view.
PDB entry 2yfr coloured by chain, top view.
Source organism: Lactobacillus johnsonii
Primary publication:
Crystal structure of inulosucrase from Lactobacillus: insights into the substrate specificity and product specificity of GH68 fructansucrases.
Pijning T, Anwar MA, Böger M, Dobruchowska JM, Leemhuis H, Kralj S, Dijkhuizen L, Dijkstra BW
J Mol Biol 412 80-93 (2011)
PMID: 21801732

Function and Biology Details

Reaction catalysed: 
Sucrose + ((2->1)-beta-D-fructosyl)(n) = glucose + ((2->1)-beta-D-fructosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-181073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inulosucrase Chain: A
Molecule details ›
Chain: A
Length: 571 amino acids
Theoretical weight: 63.85 KDa
Source organism: Lactobacillus johnsonii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q74K42 (Residues: 145-708; Coverage: 74%)
Gene names: LJ_0913, ftf, inuJ
Sequence domains: Levansucrase/Invertase
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

5 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand ACT 3 x ACT
Ligand SO4 9 x SO4
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I422
Unit cell:
a: 172.007Å b: 172.007Å c: 114.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.17
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Review of Levan polysaccharide: From a century of past experiences to future prospects.
Öner et al. (2016)
1 more

1 mention without citation

Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase.
Abaramak et al. (2021)