› 2uya

X-ray diffraction
2Å resolution

DEL162-163 mutant of Bacillus subtilis Oxalate Decarboxylase OxdC

Released:
DOI: 10.2210/pdb2uya/pdb
PDB entry 2uya coloured by chain, front view.
PDB entry 2uya coloured by chain, side view.
PDB entry 2uya coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The identity of the active site of oxalate decarboxylase and the importance of the stability of active-site lid conformations.
Just VJ, Burrell MR, Bowater L, McRobbie I, Stevenson CE, Lawson DM, Bornemann S
Biochem J 407 397-406 (2007)
PMID: 17680775

Function and Biology Details

Reaction catalysed: 
Oxalate = formate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxalate decarboxylase OxdC Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 43.38 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34714 (Residues: 1-385; Coverage: 100%)
Gene names: BSU33240, oxdC, yvrK
Sequence domains: Cupin
Structure domains: Jelly Rolls

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand TRS 1 x TRS
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 154.857Å b: 154.857Å c: 123.274Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.129 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Oxalate decarboxylase: biotechnological update and prevalence of the enzyme in filamentous fungi.
Mäkelä et al. (2010)
1 more

2 mentions without citation

Structural and immunologic characterization of Ara h 1, a major peanut allergen.
Chruszcz et al. (2011)
1 more