› 2qmu

X-ray diffraction
3.2Å resolution

Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states

Released:
DOI: 10.2210/pdb2qmu/pdb
PDB entry 2qmu coloured by chain, front view.
PDB entry 2qmu coloured by chain, side view.
PDB entry 2qmu coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states.
Yatime L, Mechulam Y, Blanquet S, Schmitt E
Proc Natl Acad Sci U S A 104 18445-50 (2007)
PMID: 18000047

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-189003 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Translation initiation factor 2 subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 45.72 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980A5 (Residues: 2-415; Coverage: 100%)
Gene names: SSO0412, eif2g
Sequence domains:
Structure domains:
Translation initiation factor 2 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 93 amino acids
Theoretical weight: 10.44 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z79 (Residues: 175-266; Coverage: 35%)
Gene names: SSO1050, aif2a, eif2a
Sequence domains: Eukaryotic translation initiation factor 2 alpha subunit
Structure domains: EIF_2_alpha
Translation initiation factor 2 subunit beta Chain: C
Molecule details ›
Chain: C
Length: 138 amino acids
Theoretical weight: 15.81 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W59 (Residues: 2-139; Coverage: 99%)
Gene names: SSO2381, aif2b, eif2b
Sequence domains: Domain found in IF2B/IF5
Structure domains: Defensin A-like

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand GDP 1 x GDP
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 118.323Å b: 118.323Å c: 161.449Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.254 0.314
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The mechanism of eukaryotic translation initiation and principles of its regulation.
Jackson et al. (2010)
12 more

7 mentions without citation

Molecular view of 43 S complex formation and start site selection in eukaryotic translation initiation.
Lorsch et al. (2010)
6 more