› 2q0l

X-ray diffraction
1.45Å resolution

Helicobacter pylori thioredoxin reductase reduced by sodium dithionite in complex with NADP+

Released:
DOI: 10.2210/pdb2q0l/pdb
PDB entry 2q0l coloured by chain, front view.
PDB entry 2q0l coloured by chain, side view.
PDB entry 2q0l coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
High-resolution structures of oxidized and reduced thioredoxin reductase from Helicobacter pylori.
Gustafsson TN, Sandalova T, Lu J, Holmgren A, Schneider G
Acta Crystallogr D Biol Crystallogr 63 833-43 (2007)
PMID: 17582174

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 33.58 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56431 (Residues: 1-311; Coverage: 100%)
Gene names: HP_0825, trxB
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 50.451Å b: 99.455Å c: 64.765Å
α: 90° β: 97.94° γ: 90°
R-values:
R R work R free
0.168 0.167 0.193
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

The thioredoxin antioxidant system.
Lu et al. (2014)
4 more