› 2j57

X-ray diffraction
2.25Å resolution

X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- quinol in complex with amicyanin.

Released:
DOI: 10.2210/pdb2j57/pdb
PDB entry 2j57 coloured by chain, front view.
PDB entry 2j57 coloured by chain, side view.
PDB entry 2j57 coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry.
Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM
J Synchrotron Radiat 14 92-8 (2007)
PMID: 17211075

Function and Biology Details

Reaction catalysed: 
Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-149613 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Amicyanin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.51 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Methylamine dehydrogenase heavy chain Chains: G, H, I, J
Molecule details ›
Chains: G, H, I, J
Length: 386 amino acids
Theoretical weight: 42.45 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P29894 (Residues: 32-417; Coverage: 99%)
Gene name: mauB
Sequence domains: Methylamine dehydrogenase heavy chain (MADH)
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Methylamine dehydrogenase light chain Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 131 amino acids
Theoretical weight: 14.21 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P22619 (Residues: 58-188; Coverage: 100%)
Gene name: mauA
Sequence domains: Methylamine dehydrogenase, L chain
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain

Ligands and Environments

1 bound ligand:
Ligand CU 4 x CU
1 modified residue:
Ligand TQQ 4 x TQQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 122.131Å b: 123.439Å c: 246.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.179 0.241

Quick links

Citations

3 review citations

Biological X-ray absorption spectroscopy (BioXAS): a valuable tool for the study of trace elements in the life sciences.
Strange et al. (2008)
2 more