› 2bhn

X-ray diffraction
3.2Å resolution

XPF from Aeropyrum pernix

Released:
DOI: 10.2210/pdb2bhn/pdb
PDB entry 2bhn coloured by chain, front view.
PDB entry 2bhn coloured by chain, side view.
PDB entry 2bhn coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition.
Newman M, Murray-Rust J, Lally J, Rudolf J, Fadden A, Knowles PP, White MF, McDonald NQ
EMBO J 24 895-905 (2005)
PMID: 15719018

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Repair endonuclease XPF Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 214 amino acids
Theoretical weight: 24.55 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9YC15 (Residues: 12-225; Coverage: 86%)
Gene name: APE_1436.1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: C2
Unit cell:
a: 210.325Å b: 42.752Å c: 118.736Å
α: 90° β: 121.42° γ: 90°
R-values:
R R work R free
0.221 0.216 0.32
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity.
Yang et al. (2006)
18 more

4 mentions without citation

DNA repair gets physical: mapping an XPA-binding site on ERCC1.
Croteau et al. (2008)
3 more