› 2alx

X-ray diffraction
2.6Å resolution

Ribonucleotide Reductase R2 from Escherichia coli in space group P6(1)22

Released:
DOI: 10.2210/pdb2alx/pdb
PDB entry 2alx coloured by chain, front view.
PDB entry 2alx coloured by chain, side view.
PDB entry 2alx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122.
Sommerhalter M, Saleh L, Bollinger JM, Rosenzweig AC
Acta Crystallogr D Biol Crystallogr 61 1649-54 (2005)
PMID: 16301799

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase 1 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 39.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69924 (Residues: 1-340; Coverage: 90%)
Gene names: JW2229, b2235, ftsB, nrdB
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand HG 4 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P6122
Unit cell:
a: 92.94Å b: 92.94Å c: 200.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.227 0.276
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The structure of the Mn4Ca2+ cluster of photosystem II and its protein environment as revealed by X-ray crystallography.
Barber et al. (2008)
2 other articles

3 mentions without citation

DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags.
Chan et al. (2004)
2 more