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Structural and biochemical identification of a novel bacterial oxidoreductase.

Loschi L, Brokx SJ, Hills TL, Zhang G, Bertero MG, Lovering AL, Weiner JH, Strynadka NC
J Biol Chem 279 50391-400 (2004)
Cited: 58 times
EuropePMC logo PMID: 15355966

Abstract

By using a bioinformatics screen of the Escherichia coli genome for potential molybdenum-containing enzymes, we have identified a novel oxidoreductase conserved in the majority of Gram-negative bacteria. The identified operon encodes for a proposed heterodimer, YedYZ in Escherichia coli, consisting of a soluble catalytic subunit termed YedY, which is likely anchored to the membrane by a heme-containing trans-membrane subunit termed YedZ. YedY is uniquely characterized by the presence of one molybdenum molybdopterin not conjugated by an additional nucleotide, and it represents the only molybdoenzyme isolated from E. coli characterized by the presence of this cofactor form. We have further characterized the catalytic subunit YedY in both the molybdenum- and tungsten-substituted forms by using crystallographic analysis. YedY is very distinct in overall architecture from all known bacterial reductases but does show some similarity with the catalytic domain of the eukaryotic chicken liver sulfite oxidase. However, the strictly conserved residues involved in the metal coordination sphere and in the substrate binding pocket of YedY are strikingly different from that of chicken liver sulfite oxidase, suggesting a catalytic activity more in keeping with a reductase than that of a sulfite oxidase. Preliminary kinetic analysis of YedY with a variety of substrates supports our proposal that YedY and its many orthologues may represent a new type of membrane-associated bacterial reductase.

Reviews - 1xdq mentioned but not cited (6)

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Articles - 1xdq mentioned but not cited (4)

  1. Electrochemical evidence that pyranopterin redox chemistry controls the catalysis of YedY, a mononuclear Mo enzyme. Adamson H, Simonov AN, Kierzek M, Rothery RA, Weiner JH, Bond AM, Parkin A. Proc Natl Acad Sci U S A 112 14506-14511 (2015)
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  4. research-article Control of Biofilm Formation by an Agrobacterium tumefaciens Pterin-Binding Periplasmic Protein Conserved Among Pathogenic Bacteria. Greenwich JL, Eagan JL, Feirer N, Boswinkle K, Minasov G, Shuvalova L, Inniss NL, Raghavaiah J, Ghosh AK, Satchell KJF, Allen KD, Fuqua C. bioRxiv 2023.11.18.567607 (2023)


Reviews citing this publication (11)

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  15. Novel mechanism for scavenging of hypochlorite involving a periplasmic methionine-rich Peptide and methionine sulfoxide reductase. Melnyk RA, Youngblut MD, Clark IC, Carlson HK, Wetmore KM, Price MN, Iavarone AT, Deutschbauer AM, Arkin AP, Coates JD. mBio 6 e00233-15 (2015)
  16. A Two-component NADPH Oxidase (NOX)-like System in Bacteria Is Involved in the Electron Transfer Chain to the Methionine Sulfoxide Reductase MsrP. Juillan-Binard C, Picciocchi A, Andrieu JP, Dupuy J, Petit-Hartlein I, Caux-Thang C, Vivès C, Nivière V, Fieschi F. J Biol Chem 292 2485-2494 (2017)
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  19. Identification of a Salmonella ancillary copper detoxification mechanism by a comparative analysis of the genome-wide transcriptional response to copper and zinc excess. Pontel LB, Scampoli NL, Porwollik S, Checa SK, McClelland M, Soncini FC. Microbiology (Reading) 160 1659-1669 (2014)
  20. What matters in chronic Burkholderia cenocepacia infection in cystic fibrosis: Insights from comparative genomics. Nunvar J, Capek V, Fiser K, Fila L, Drevinek P. PLoS Pathog 13 e1006762 (2017)
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  26. Genome analysis of Pseudoalteromonas flavipulchra JG1 reveals various survival advantages in marine environment. Yu M, Tang K, Liu J, Shi X, Gulder TA, Zhang XH. BMC Genomics 14 707 (2013)
  27. Microarray transcriptional profiling of Arctic Mesorhizobium strain N33 at low temperature provides insights into cold adaption strategies. Ghobakhlou AF, Johnston A, Harris L, Antoun H, Laberge S. BMC Genomics 16 383 (2015)
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  30. Population differentiation of Rhodobacteraceae along with coral compartments. Luo D, Wang X, Feng X, Tian M, Wang S, Tang SL, Ang P, Yan A, Luo H. ISME J 15 3286-3302 (2021)
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  34. Short communication: Homologous expression of recombinant and native thurincin H in an engineered natural producer. Wang G, Manns DC, Guron GK, Churey JJ, Worobo RW. J Dairy Sci 97 4120-4126 (2014)
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  36. Exclusively membrane-inserted state of an uncleavable Tat precursor protein suggests lateral transfer into the bilayer from the translocon. Ren C, Patel R, Robinson C. FEBS J 280 3354-3364 (2013)
  37. A novel bacterial sulfite dehydrogenase that requires three c-type cytochromes for electron transfer. Sun W, Xu Y, Liang Y, Yu Q, Gao H. Appl Environ Microbiol 89 e0110823 (2023)


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