› 1x1s

X-ray diffraction
2.2Å resolution

Crystal structure of M-Ras in complex with GppNHp

Released:
DOI: 10.2210/pdb1x1s/pdb
PDB entry 1x1s coloured by chain, front view.
PDB entry 1x1s coloured by chain, side view.
PDB entry 1x1s coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of M-Ras reveals a GTP-bound "off" state conformation of Ras family small GTPases.
Ye M, Shima F, Muraoka S, Liao J, Okamoto H, Yamamoto M, Tamura A, Yagi N, Ueki T, Kataoka T
J Biol Chem 280 31267-75 (2005)
PMID: 15994326

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein M-Ras Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08989 (Residues: 1-178; Coverage: 86%)
Gene names: Mras, Xras
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P622
Unit cell:
a: 109.711Å b: 109.711Å c: 69.426Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.256
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
8 more

8 mentions without citation

Structural basis for conformational dynamics of GTP-bound Ras protein.
Shima et al. (2010)
7 more