PDBe 1sgf

X-ray diffraction
3.15Å resolution

CRYSTAL STRUCTURE OF 7S NGF: A COMPLEX OF NERVE GROWTH FACTOR WITH FOUR BINDING PROTEINS (SERINE PROTEINASES)

Released:

Function and Biology Details

Reaction catalysed:
Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kallikrein 1-related peptidase-like b4 Chains: A, X
Molecule details ›
Chains: A, X
Length: 240 amino acids
Theoretical weight: 26.78 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00757 (Residues: 17-256; Coverage: 100%)
Gene names: Klk-4, Klk1b4, Klk4, Ngfa
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Beta-nerve growth factor Chains: B, Y
Molecule details ›
Chains: B, Y
Length: 118 amino acids
Theoretical weight: 13.28 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01139 (Residues: 122-239; Coverage: 53%)
Gene names: Ngf, Ngfb
Sequence domains: Nerve growth factor family
Structure domains: Cystine-knot cytokines
Kallikrein 1-related peptidase b3 Chains: G, Z
Molecule details ›
Chains: G, Z
Length: 237 amino acids
Theoretical weight: 26.43 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00756 (Residues: 25-261; Coverage: 98%)
Gene names: Klk-3, Klk1b3, Klk3, Ngfg
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG
Spacegroup: P212121
Unit cell:
a: 95.68Å b: 96.59Å c: 147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.282