› 1pmj

X-ray diffraction
1.55Å resolution

Crystal structure of Caldicellulosiruptor saccharolyticus CBM27-1

Released:
DOI: 10.2210/pdb1pmj/pdb
PDB entry 1pmj coloured by chain, front view.
PDB entry 1pmj coloured by chain, side view.
PDB entry 1pmj coloured by chain, top view.
Source organism: Caldicellulosiruptor saccharolyticus
Primary publication:
High-resolution crystal structures of Caldicellulosiruptor strain Rt8B.4 carbohydrate-binding module CBM27-1 and its complex with mannohexaose.
Roske Y, Sunna A, Pfeil W, Heinemann U
J Mol Biol 340 543-54 (2004)
PMID: 15210353

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-1,4-mannanase Chain: X
Molecule details ›
Chain: X
Length: 185 amino acids
Theoretical weight: 20.81 KDa
Source organism: Caldicellulosiruptor saccharolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P77847 (Residues: 36-220; Coverage: 20%)
Gene name: manA
Sequence domains: Carbohydrate binding module 27
Structure domains: Galactose-binding domain-like

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand EDO 2 x EDO
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 54.988Å b: 54.988Å c: 115.777Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.124 0.174
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Advances in molecular engineering of carbohydrate-binding modules.
Armenta et al. (2017)
1 more

2 mentions without citation

Modular hyperthermostable bacterial endo-β-1,4-mannanase: molecular shape, flexibility and temperature-dependent conformational changes.
da Silva et al. (2014)
1 more