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Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase.

Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW
Proc Natl Acad Sci U S A 99 15375-80 (2002)
Related entries: 1n1b, 1n1z, 1n21, 1n22, 1n23, 1n24

Cited: 160 times
EuropePMC logo PMID: 12432096

Abstract

The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a metal-requiring monoterpene cyclase from Salvia officinalis, is reported at 2.0-A resolution. Each monomer contains two alpha-helical domains: the C-terminal domain catalyzes the cyclization of geranyl diphosphate, orienting and stabilizing multiple reactive carbocation intermediates; the N-terminal domain has no clearly defined function, although its N terminus caps the active site in the C-terminal domain during catalysis. Structures of complexes with aza analogues of substrate and carbocation intermediates, as well as complexes with pyrophosphate and bornyl diphosphate, provide "snapshots" of the terpene cyclization cascade.

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