› 1jwn

X-ray diffraction
2.1Å resolution

Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.

Released:
DOI: 10.2210/pdb1jwn/pdb
PDB entry 1jwn coloured by chain, front view.
PDB entry 1jwn coloured by chain, side view.
PDB entry 1jwn coloured by chain, top view.
Source organism: Anadara inaequivalvis
Primary publication:
Restricting the ligand-linked heme movement in Scapharca dimeric hemoglobin reveals tight coupling between distal and proximal contributions to cooperativity.
Knapp JE, Gibson QH, Cushing L, Royer WE
Biochemistry 40 14795-805 (2001)
PMID: 11732898

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 16 KDa
Source organism: Anadara inaequivalvis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02213 (Residues: 1-146; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand CMO 4 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 83.68Å b: 44.14Å c: 87.28Å
α: 90° β: 115.42° γ: 90°
R-values:
R R work R free
0.215 0.211 0.247
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Allosteric hemoglobin assembly: diversity and similarity.
Royer et al. (2005)
3 more

4 mentions without citation

Simple mechanisms for the evolution of protein complexity.
Pillai et al. (2022)
3 more