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NMR structures of ferredoxin chloroplastic transit peptide from Chlamydomonas reinhardtii promoted by trifluoroethanol in aqueous solution.

Lancelin JM, Bally I, Arlaud GJ, Blackledge M, Gans P, Stein M, Jacquot JP
FEBS Lett 343 261-6 (1994)
Cited: 25 times
EuropePMC logo PMID: 8174712

Abstract

The 32-amino acid transit peptide of the unicellular green alga Chlamydomonas reinhardtii ferredoxin has been synthesized and analysed by NMR spectroscopy and circular dichroism. The results show that while the peptide is unstructured in water, it undergoes an alpha-helix formation from residue 3 to 13 in a 30:70 molar-ratio mixture of 2,2,2-trifluoroethanol. The remainder of the peptide is still unstructured in CF3CD2OD/H2O mixtures, but is distributed on a side opposite to a hydrophobic ridge formed by Met5, Phe9 and Val13 on the induced alpha-helix. The NMR structures driven by 2,2,2-trifluoroethanol in aqueous solution, are discussed in terms of potent interactions with the chloroplast envelope and its translocation molecular machinery.

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Reviews citing this publication (5)

  1. Chloroplast transit peptides: structure, function and evolution. Bruce BD. Trends Cell Biol 10 440-447 (2000)
  2. The paradox of plastid transit peptides: conservation of function despite divergence in primary structure. Bruce BD. Biochim Biophys Acta 1541 2-21 (2001)
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Articles citing this publication (17)

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  2. A model for the interaction of trifluoroethanol with peptides and proteins. Rajan R, Balaram P. Int J Pept Protein Res 48 328-336 (1996)
  3. Identification of a Hsp70 recognition domain within the rubisco small subunit transit peptide. Ivey RA, Subramanian C, Bruce BD. Plant Physiol 122 1289-1299 (2000)
  4. Chlamydomonas reinhardtii thioredoxins: structure of the genes coding for the chloroplastic m and cytosolic h isoforms; expression in Escherichia coli of the recombinant proteins, purification and biochemical properties. Stein M, Jacquot JP, Jeannette E, Decottignies P, Hodges M, Lancelin JM, Mittard V, Schmitter JM, Miginiac-Maslow M. Plant Mol Biol 28 487-503 (1995)
  5. The structural flexibility of the preferredoxin transit peptide. Wienk HL, Czisch M, de Kruijff B. FEBS Lett 453 318-326 (1999)
  6. A coil-helix instead of a helix-coil motif can be induced in a chloroplast transit peptide from Chlamydomonas reinhardtii. Krimm I, Gans P, Hernandez JF, Arlaud GJ, Lancelin JM. Eur J Biochem 265 171-180 (1999)
  7. Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast. Muto M, Henry RE, Mayfield SP. BMC Biotechnol 9 26 (2009)
  8. Plasmodium falciparum apicoplast transit peptides are unstructured in vitro and during apicoplast import. Gallagher JR, Matthews KA, Prigge ST. Traffic 12 1124-1138 (2011)
  9. Effects of a tryptophanyl substitution on the structure and antimicrobial activity of C-terminally truncated gaegurin 4. Won HS, Park SH, Kim HE, Hyun B, Kim M, Lee BJ, Lee BJ. Eur J Biochem 269 4367-4374 (2002)
  10. Import determinants of organelle-specific and dual targeting peptides of mitochondria and chloroplasts in Arabidopsis thaliana. Ge C, Spånning E, Glaser E, Wieslander A. Mol Plant 7 121-136 (2014)
  11. Solution structure and membrane-binding property of the N-terminal tail domain of human annexin I. Yoon MK, Park SH, Won HS, Na DS, Lee BJ. FEBS Lett 484 241-245 (2000)
  12. NMR structures of a mitochondrial transit peptide from the green alga Chlamydomonas reinhardtii. Lancelin JM, Gans P, Bouchayer E, Bally I, Arlaud GJ, Jacquot JP. FEBS Lett 391 203-208 (1996)
  13. Identification, cDNA sequence and deduced amino acid sequence of the mitochondrial Rieske iron-sulfur protein from the green alga Chlamydomonas reinhardtii. Implications for protein targeting and subunit interaction. Atteia A, Franzén LG. Eur J Biochem 237 792-799 (1996)
  14. A mammalian cytochrome fused to a chloroplast transit peptide is a functional haemoprotein and is imported into isolated chloroplasts. Liu YY, Kaderbhai N, Kaderbhai MA. Biochem J 351 Pt 2 377-384 (2000)
  15. Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles. Garrido C, Caspari OD, Choquet Y, Wollman FA, Lafontaine I. Cells 9 (2020)
  16. Architecture of chloroplast TOC-TIC translocon supercomplex. Liu H, Li A, Rochaix JD, Liu Z. Nature 615 349-357 (2023)
  17. Conformation study of HA(306-318) antigenic peptide of the haemagglutinin influenza virus protein. Bertrand A, Brito RM, Alix AJ, Lancelin JM, Carvalho RA, Geraldes CF, Lakhdar-Ghazal F. Spectrochim Acta A Mol Biomol Spectrosc 65 711-718 (2006)


Related citations provided by authors (1)

  1. A cDNA clone encoding Chlamydomonas reinhardtii preferredoxin.. Stein M, Jacquot JP, Miginiac-Maslow M Plant Physiol 102 1349-50 (1993)

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