› 1a55

X-ray diffraction
2.4Å resolution

PHOSPHATE-BINDING PROTEIN MUTANT A197C

Released:
DOI: 10.2210/pdb1a55/pdb
PDB entry 1a55 coloured by chain, front view.
PDB entry 1a55 coloured by chain, side view.
PDB entry 1a55 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate.
Hirshberg M, Henrick K, Haire LL, Vasisht N, Brune M, Corrie JE, Webb MR
Biochemistry 37 10381-5 (1998)
PMID: 9671506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-binding protein PstS Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG82 (Residues: 26-346; Coverage: 100%)
Gene names: JW3706, b3728, phoS, pstS
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand 2HP 1 x 2HP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 42.04Å b: 64.161Å c: 124.353Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.227
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
8 more