PDBe 1ign

X-ray diffraction
2.25Å resolution

DNA-BINDING DOMAIN OF RAP1 IN COMPLEX WITH TELOMERIC DNA SITE

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding protein RAP1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 28.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11938 (Residues: 353-598; Coverage: 30%)
Gene names: GRF1, N1310, RAP1, TUF1, YNL216W
Sequence domains:
Structure domains: Homeodomain-like
DNA (5'-D(*CP*CP*GP*CP*AP*CP*AP*CP*CP*CP*AP*CP*AP*CP*AP*CP*C P*AP*G)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 19 nucleotides
Theoretical weight: 5.67 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*CP*TP*GP*GP*TP*GP*TP*GP*TP*GP*GP*GP*TP*GP*TP*G P*CP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 19 nucleotides
Theoretical weight: 5.94 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P31
Unit cell:
a: 90.61Å b: 90.61Å c: 80.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.294
Expression systems:
  • Escherichia coli
  • Not provided