› 7lol

X-ray diffraction
1.8Å resolution

The structure of Agmatinase from E. Coli at 1.8 A displaying urea and agmatine

Released:
DOI: 10.2210/pdb7lol/pdb
PDB entry 7lol coloured by chain, front view.
PDB entry 7lol coloured by chain, side view.
PDB entry 7lol coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal Structure of Escherichia coli Agmatinase: Catalytic Mechanism and Residues Relevant for Substrate Specificity.
Maturana P, Orellana MS, Herrera SM, Martínez I, Figueroa M, Martínez-Oyanedel J, Castro-Fernandez V, Uribe E
OpenAccess logo Int J Mol Sci 22 (2021)
PMID: 33946272

Function and Biology Details

Reaction catalysed: 
Agmatine + H(2)O = putrescine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Agmatinase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60651 (Residues: 1-306; Coverage: 100%)
Gene names: JW2904, b2937, speB
Sequence domains: Arginase family

Ligands and Environments

4 bound ligands:
Ligand AG2 1 x AG2
Ligand MN 3 x MN
Ligand URE 1 x URE
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R32
Unit cell:
a: 81.746Å b: 81.746Å c: 207.436Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.195
Expression system: Escherichia coli BL21

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Citations

1 citations in other articles

A Methylotrophic Bacterium Growing with the Antidiabetic Drug Metformin as Its Sole Carbon, Nitrogen and Energy Source.
Chaignaud et al. (2022)

2 mentions without citation

Crystal Structure of Escherichia coli Agmatinase: Catalytic Mechanism and Residues Relevant for Substrate Specificity.
Maturana et al. (2021)
1 more