› 6ufh

X-ray diffraction
3.1Å resolution

Co-crystal structure of M. tuberculosis ileS T-box in complex with tRNA-3'-2'3'cyclic phosphate

Released:
DOI: 10.2210/pdb6ufh/pdb
PDB entry 6ufh coloured by chain, front view.
PDB entry 6ufh coloured by chain, side view.
PDB entry 6ufh coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators.
Battaglia RA, Grigg JC, Ke A
Nat Struct Mol Biol 26 1106-1113 (2019)
PMID: 31740853

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195830 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (77-MER) Chain: B
Molecule details ›
Chain: B
Length: 77 nucleotides
Theoretical weight: 24.92 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided
Sequence domains: tRNA
RNA (167-MER) Chain: A
Molecule details ›
Chain: A
Length: 167 nucleotides
Theoretical weight: 54.45 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
3 modified residues:
Ligand A23 1 x A23
Ligand GTP 1 x GTP
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 76.276Å b: 61.43Å c: 122.52Å
α: 90° β: 93.97° γ: 90°
R-values:
R R work R free
0.225 0.22 0.269
Expression system: Not provided

Quick links

Citations

7 review citations

Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.
Zhang J. (2020)
6 more

1 mention without citation

Structural basis for tRNA decoding and aminoacylation sensing by T-box riboregulators.
Battaglia et al. (2019)