› 6uc7

X-ray diffraction
1.8Å resolution

Structure of guanine riboswitch bound to N2-acetyl guanine

Released:
DOI: 10.2210/pdb6uc7/pdb
PDB entry 6uc7 coloured by chain, front view.
PDB entry 6uc7 coloured by chain, side view.
PDB entry 6uc7 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
High Affinity Binding of N2-Modified Guanine Derivatives Significantly Disrupts the Ligand Binding Pocket of the Guanine Riboswitch.
Matyjasik MM, Hall SD, Batey RT
OpenAccess logo Molecules 25 (2020)
PMID: 32414072

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197138 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
guanine riboswitch Chain: B
Molecule details ›
Chain: B
Length: 67 nucleotides
Theoretical weight: 21.51 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
Sequence domains: Purine riboswitch aptamer

Ligands and Environments

2 bound ligands:
Ligand NCO 8 x NCO
Ligand Q44 1 x Q44
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 132.107Å b: 35.082Å c: 41.606Å
α: 90° β: 90.99° γ: 90°
R-values:
R R work R free
0.184 not available 0.209
Expression system: Not provided

Quick links

Citations

2 review citations

Riboswitches as Drug Targets for Antibiotics.
Panchal et al. (2021)
1 more

1 mention without citation

SILCS-RNA: Toward a Structure-Based Drug Design Approach for Targeting RNAs with Small Molecules.
Kognole et al. (2022)