› 6sos

X-ray diffraction
2.2Å resolution

Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide

Released:
DOI: 10.2210/pdb6sos/pdb
PDB entry 6sos coloured by chain, front view.
PDB entry 6sos coloured by chain, side view.
PDB entry 6sos coloured by chain, top view.
Source organisms:
Primary publication:
The Role of Changing Loop Conformations in Streptavidin Versions Engineered for High-affinity Binding of the Strep-tag II Peptide.
Schmidt TGM, Eichinger A, Schneider M, Bonet L, Carl U, Karthaus D, Theobald I, Skerra A
J Mol Biol 433 166893 (2021)
PMID: 33639211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149740 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.35 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-163; Coverage: 79%)
Sequence domains: Avidin family
Twin-Strep-tag peptide Chains: P, R
Molecule details ›
Chains: P, R
Length: 32 amino acids
Theoretical weight: 3.17 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand BE2 2 x BE2

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 101.769Å b: 101.769Å c: 118.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 citation in other articles

Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether-Bond-Forming Sactisynthase.
Ali et al. (2022)
3 more