› 6fqd

X-ray diffraction
2.1Å resolution

Escherichia Coli Signal Recognition Particle Receptor FtsY NGdN1

Released:
DOI: 10.2210/pdb6fqd/pdb
PDB entry 6fqd coloured by chain, front view.
PDB entry 6fqd coloured by chain, side view.
PDB entry 6fqd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Co-translational Folding Intermediate Dictates Membrane Targeting of the Signal Recognition Particle Receptor.
Karniel A, Mrusek D, Steinchen W, Dym O, Bange G, Bibi E
J Mol Biol 430 1607-1620 (2018)
PMID: 29704493

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145174 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle receptor FtsY Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 31.23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10121 (Residues: 221-497; Coverage: 56%)
Gene names: JW3429, b3464, ftsY
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I4
Unit cell:
a: 110.963Å b: 110.963Å c: 110.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Cotranslational Folding of Proteins on the Ribosome.
Liutkute et al. (2020)
2 more