› 5thy

X-ray diffraction
2.09Å resolution

Crystal structure of SeMet-Substituted CurJ carbon methyltransferase

Released:
DOI: 10.2210/pdb5thy/pdb
PDB entry 5thy coloured by chain, front view.
PDB entry 5thy coloured by chain, side view.
PDB entry 5thy coloured by chain, top view.
Source organism: Moorena producens 3L
Primary publication:
Domain Organization and Active Site Architecture of a Polyketide Synthase C-methyltransferase.
Skiba MA, Sikkema AP, Fiers WD, Gerwick WH, Sherman DH, Aldrich CC, Smith JL
ACS Chem Biol 11 3319-3327 (2016)
PMID: 27723289

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CurJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 45.46 KDa
Source organism: Moorena producens 3L
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F4Y426 (Residues: 1269-1649; Coverage: 17%)
Gene name: LYNGBM3L_74460
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SAH 2 x SAH
Ligand GDS 1 x GDS
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 48.35Å b: 130.39Å c: 62.83Å
α: 90° β: 111.98° γ: 90°
R-values:
R R work R free
0.171 0.168 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
4 more

3 mentions without citation

Domain Organization and Active Site Architecture of a Polyketide Synthase C-methyltransferase.
Skiba et al. (2016)
2 more