› 5ls4

X-ray diffraction
1.47Å resolution

Mopeia virus exonuclease domain complexed with Calcium

Released:
DOI: 10.2210/pdb5ls4/pdb
PDB entry 5ls4 coloured by chain, front view.
PDB entry 5ls4 coloured by chain, side view.
PDB entry 5ls4 coloured by chain, top view.
Source organism: Mopeia virus AN20410
Primary publication:
Activity inhibition and crystal polymorphism induced by active-site metal swapping.
Yekwa E, Khourieh J, Canard B, Papageorgiou N, Ferron F
Acta Crystallogr D Struct Biol 73 641-649 (2017)
PMID: 28777079

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.56 KDa
Source organism: Mopeia virus AN20410
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S581 (Residues: 365-570; Coverage: 36%)
Gene names: N, NP
Sequence domains: Arenavirus nucleocapsid C-terminal domain

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand ZN 1 x ZN
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 131.962Å b: 37.913Å c: 48.986Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.172 0.17 0.197
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Brothers in Arms: Structure, Assembly and Function of Arenaviridae Nucleoprotein.
Papageorgiou et al. (2020)
1 more

1 mention without citation

Inhibition of Arenaviridae nucleoprotein exonuclease by bisphosphonate.
Nguyen et al. (2022)