› 5enz

X-ray diffraction
1.91Å resolution

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 43.69 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9REV4 (Residues: 1-375; Coverage: 100%)
Gene names: AC807_2437, C0100_008625, CFN12_11545, DQV00_04090, EP54_10875, EQ90_06770, ERS092965_02425, ERS195417_02090, G0V59_07625, G0Z31_05865, G6W84_12025, GO814_01085, GO942_06315, GQX37_08560, GQX52_10550, GZ156_04915, HT539_11440, M1K003_2228, NCTC10702_03339, NCTC13131_01446, SAMEA1029519_02146, SAMEA4008627_02436, SAMEA4552975_02490, mnaA, mnaA_1, mnaA_2, mnaA_3, wecB
Sequence domains: UDP-N-acetylglucosamine 2-epimerase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

4 bound ligands:
Ligand UDP 2 x UDP
Ligand SO4 4 x SO4
Ligand TRS 2 x TRS
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 54.19Å b: 82.78Å c: 170.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Targeting the Holy Triangle of Quorum Sensing, Biofilm Formation, and Antibiotic Resistance in Pathogenic Bacteria.
Sionov et al. (2022)
3 more

3 mentions without citation

Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets.
Mann et al. (2016)
2 more