› 5v9h

X-ray diffraction
2.15Å resolution

Structure of the H477R variant of rat cytosolic PEPCK in complex with phosphoglycolate and GDP.

Released:
DOI: 10.2210/pdb5v9h/pdb
PDB entry 5v9h coloured by chain, front view.
PDB entry 5v9h coloured by chain, side view.
PDB entry 5v9h coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Asymmetric Anchoring Is Required for Efficient Ω-Loop Opening and Closing in Cytosolic Phosphoenolpyruvate Carboxykinase.
Cui DS, Broom A, Mcleod MJ, Meiering EM, Holyoak T
Biochemistry 56 2106-2115 (2017)
PMID: 28345895

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 622 amino acids
Theoretical weight: 69.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand 1PE 1 x 1PE
Ligand MN 6 x MN
Ligand NA 2 x NA
Ligand GDP 2 x GDP
Ligand PGA 2 x PGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 60.48Å b: 119.433Å c: 88.166Å
α: 90° β: 105.3° γ: 90°
R-values:
R R work R free
0.244 0.241 0.285
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Dynamic Acetylation of Phosphoenolpyruvate Carboxykinase Toggles Enzyme Activity between Gluconeogenic and Anaplerotic Reactions.
Latorre-Muro et al. (2018)
3 more