› 5iew

Solution NMR

NMR Structures Show Unwinding of the GCN4p Coiled Coil Superhelix Accompanying Disruption of Ion Pairs at Acidic pH

Released:
DOI: 10.2210/pdb5iew/pdb
PDB entry 5iew coloured by chain, ensemble of 20 models, front view.
PDB entry 5iew coloured by chain, ensemble of 20 models, side view.
PDB entry 5iew coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Nuclear Magnetic Resonance Structures of GCN4p Are Largely Conserved When Ion Pairs Are Disrupted at Acidic pH but Show a Relaxation of the Coiled Coil Superhelix.
Kaplan AR, Brady MR, Maciejewski MW, Kammerer RA, Alexandrescu AT
Biochemistry 56 1604-1619 (2017)
PMID: 28230348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 33 amino acids
Theoretical weight: 3.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 250-280; Coverage: 11%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 45%
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Conformational Dynamics of Asparagine at Coiled-Coil Interfaces.
Thomas et al. (2017)
2 more

2 mentions without citation

Nuclear Magnetic Resonance Structures of GCN4p Are Largely Conserved When Ion Pairs Are Disrupted at Acidic pH but Show a Relaxation of the Coiled Coil Superhelix.
Kaplan et al. (2017)
1 more