› 5c9h

X-ray diffraction
3Å resolution

Structural Basis of Template Boundary Definition in Tetrahymena Telomerase

Released:
DOI: 10.2210/pdb5c9h/pdb
PDB entry 5c9h coloured by chain, front view.
PDB entry 5c9h coloured by chain, side view.
PDB entry 5c9h coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structural basis of template-boundary definition in Tetrahymena telomerase.
Jansson LI, Akiyama BM, Ooms A, Lu C, Rubin SM, Stone MD
OpenAccess logo Nat Struct Mol Biol 22 883-8 (2015)
PMID: 26436828

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131269 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Telomerase reverse transcriptase Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 36.77 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: O77448 (Residues: 217-516; Coverage: 27%)
Gene names: TERT, TTHERM_00112560
Sequence domains: Telomerase ribonucleoprotein complex - RNA binding domain
Structure domains: Topoisomerase I; Chain A, domain 4
RNA (5'-R(P*AP*GP*AP*AP*CP*UP*GP*UP*CP*A)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 13 nucleotides
Theoretical weight: 4.1 KDa
Source organism: Tetrahymena thermophila
Expression system: Not provided
RNA (5'-R(P*UP*CP*AP*UP*UP*CP*AP*GP*UP*UP*CP*U)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Tetrahymena thermophila
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 58.53Å b: 117.77Å c: 131.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.239 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Roles of telomeres and telomerase in cancer, and advances in telomerase-targeted therapies.
Jafri et al. (2016)
7 more

9 mentions without citation

Progress in Human and Tetrahymena Telomerase Structure Determination.
Chan et al. (2017)
8 more