› 4wr5

X-ray diffraction
1.93Å resolution

Crystal Structure of GST Mutated with Halogenated Tyrosine (7cGST-1)

Released:
DOI: 10.2210/pdb4wr5/pdb
PDB entry 4wr5 coloured by chain, front view.
PDB entry 4wr5 coloured by chain, side view.
PDB entry 4wr5 coloured by chain, top view.
Source organism: Schistosoma japonicum
Primary publication:
Protein stabilization utilizing a redefined codon.
Ohtake K, Yamaguchi A, Mukai T, Kashimura H, Hirano N, Haruki M, Kohashi S, Yamagishi K, Murayama K, Tomabechi Y, Itagaki T, Akasaka R, Kawazoe M, Takemoto C, Shirouzu M, Yokoyama S, Sakamoto K
Sci Rep 5 9762 (2015)
PMID: 25985257

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase class-mu 26 kDa isozyme Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 28.38 KDa
Source organism: Schistosoma japonicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08515 (Residues: 2-218; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand 3CT 7 x 3CT

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P6522
Unit cell:
a: 63.7Å b: 63.7Å c: 224.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Playing with the Molecules of Life.
Young et al. (2018)
7 more

1 mention without citation

Protein stabilization utilizing a redefined codon.
Ohtake et al. (2015)