› 4orn

X-ray diffraction
1.71Å resolution

Blue Fluorescent Protein mKalama1

Released:
DOI: 10.2210/pdb4orn/pdb
PDB entry 4orn coloured by chain, front view.
PDB entry 4orn coloured by chain, side view.
PDB entry 4orn coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Hidden photoinduced reactivity of the blue fluorescent protein mKalama1.
Vegh RB, Bloch DA, Bommarius AS, Verkhovsky M, Pletnev S, Iwaï H, Bochenkova AV, Solntsev KM
Phys Chem Chem Phys 17 12472-85 (2015)
PMID: 25805012

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.01 KDa
Source organism: Aequorea victoria
Expression system: Not provided
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand MES 2 x MES
Ligand SO4 6 x SO4
Ligand CL 4 x CL
2 modified residues:
Ligand GYS 2 x GYS
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 80.51Å b: 83.46Å c: 92.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.183
Expression system: Not provided

Quick links

Citations

2 review citations

Novel uses of fluorescent proteins.
Mishin et al. (2015)
1 more

1 mention without citation

BioBits™ Bright: A fluorescent synthetic biology education kit.
Stark et al. (2018)