› 4nfz

X-ray diffraction
2.7Å resolution

Crystal structure of polymerase subunit PA N-terminal endonuclease domain from bat-derived influenza virus H17N10

Released:
DOI: 10.2210/pdb4nfz/pdb
PDB entry 4nfz coloured by chain, front view.
PDB entry 4nfz coloured by chain, side view.
PDB entry 4nfz coloured by chain, top view.
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Primary publication:
The N-terminal domain of PA from bat-derived influenza-like virus H17N10 has endonuclease activity.
Tefsen B, Lu G, Zhu Y, Haywood J, Zhao L, Deng T, Qi J, Gao GF
J Virol 88 1935-41 (2014)
PMID: 24284327

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-124828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 214 amino acids
Theoretical weight: 25.5 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Escherichia coli
UniProt:
  • Canonical: H6QM92 (Residues: 1-206; Coverage: 29%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

1 bound ligand:
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 74.3Å b: 74.3Å c: 401.159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.24 0.278
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Learning from structure-based drug design and new antivirals targeting the ribonucleoprotein complex for the treatment of influenza.
Monod et al. (2015)
3 more

2 mentions without citation

The Influenza Virus Polymerase Complex: An Update on Its Structure, Functions, and Significance for Antiviral Drug Design.
Stevaert et al. (2016)
1 more