› 4eet

X-ray diffraction
1.2Å resolution

Crystal structure of iLOV

Released:
DOI: 10.2210/pdb4eet/pdb
PDB entry 4eet coloured by chain, front view.
PDB entry 4eet coloured by chain, side view.
PDB entry 4eet coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural tuning of the fluorescent protein iLOV for improved photostability.
Christie JM, Hitomi K, Arvai AS, Hartfield KA, Mettlen M, Pratt AJ, Tainer JA, Getzoff ED
J Biol Chem 287 22295-304 (2012)
PMID: 22573334

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phototropin-2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 115 amino acids
Theoretical weight: 13.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P93025 (Residues: 385-496; Coverage: 12%)
Gene names: At5g58140, CAV1, K21L19.6, KIN7, NPL1, PHOT2
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P41212
Unit cell:
a: 46.474Å b: 46.474Å c: 207.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.211
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Super-resolution in solution X-ray scattering and its applications to structural systems biology.
Rambo et al. (2013)
15 more

3 mentions without citation

Plasmonic photocatalyst-like fluorescent proteins for generating reactive oxygen species.
Leem et al. (2018)
2 more