› 4zvc

X-ray diffraction
1.5Å resolution

Crystal structure of MID domain of the E. coli DosC - form I

Released:
DOI: 10.2210/pdb4zvc/pdb
PDB entry 4zvc coloured by chain, front view.
PDB entry 4zvc coloured by chain, side view.
PDB entry 4zvc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural analysis of an oxygen-regulated diguanylate cyclase.
Tarnawski M, Barends TR, Schlichting I
Acta Crystallogr D Biol Crystallogr 71 2158-77 (2015)
PMID: 26527135

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate cyclase DosC Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 14.68 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA89 (Residues: 173-298; Coverage: 27%)
Gene names: JW5241, b1490, dgcO, dosC, yddV
Sequence domains: DosC CZB-like middle domain

Ligands and Environments

1 bound ligand:
Ligand BEZ 1 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 49.22Å b: 43.57Å c: 52.44Å
α: 90° β: 100.94° γ: 90°
R-values:
R R work R free
0.15 0.147 0.189
Expression system: Escherichia coli

Quick links

Citations

4 review citations

C-di-GMP Synthesis: Structural Aspects of Evolution, Catalysis and Regulation.
Schirmer T. (2016)
3 more

2 mentions without citation

Mechanism and Role of Globin-Coupled Sensor Signalling.
Walker et al. (2017)
1 more