› 4ykg

X-ray diffraction
2.4Å resolution

Crystal Structure of the Alkylhydroperoxide Reductase subunit F (AhpF) with NAD+ from Escherichia coli

Released:
DOI: 10.2210/pdb4ykg/pdb
PDB entry 4ykg coloured by chain, front view.
PDB entry 4ykg coloured by chain, side view.
PDB entry 4ykg coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystallographic and solution studies of NAD(+)- and NADH-bound alkylhydroperoxide reductase subunit F (AhpF) from Escherichia coli provide insight into sequential enzymatic steps.
Kamariah N, Manimekalai MS, Nartey W, Eisenhaber F, Eisenhaber B, Grüber G
Biochim Biophys Acta 1847 1139-52 (2015)
PMID: 26092085

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase subunit F Chain: A
Molecule details ›
Chain: A
Length: 521 amino acids
Theoretical weight: 56.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P35340 (Residues: 1-521; Coverage: 100%)
Gene names: JW0599, ahpF, b0606
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand FAD 1 x FAD
Ligand NAD 1 x NAD
Ligand SO4 9 x SO4
Ligand CD 1 x CD
Ligand GOL 6 x GOL
Ligand PEG 2 x PEG
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 106.882Å b: 59.576Å c: 121.508Å
α: 90° β: 111.4° γ: 90°
R-values:
R R work R free
0.224 0.221 0.285
Expression system: Escherichia coli

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Citations

10 citation in other articles

Transition steps in peroxide reduction and a molecular switch for peroxide robustness of prokaryotic peroxiredoxins.
Kamariah et al. (2016)
9 more