› 4lqu

X-ray diffraction
1.6Å resolution

1.60A resolution crystal structure of a superfolder green fluorescent protein (W57G) mutant

Released:
DOI: 10.2210/pdb4lqu/pdb
PDB entry 4lqu coloured by chain, front view.
PDB entry 4lqu coloured by chain, side view.
PDB entry 4lqu coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
The designability of protein switches by chemical rescue of structure: mechanisms of inactivation and reactivation.
Xia Y, DiPrimio N, Keppel TR, Vo B, Fraser K, Battaile KP, Egan C, Bystroff C, Lovell S, Weis DD, Anderson JC, Karanicolas J
J Am Chem Soc 135 18840-9 (2013)
PMID: 24313858

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 27.68 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 96.065Å b: 97.252Å c: 98.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.182
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Allostery in Its Many Disguises: From Theory to Applications.
Wodak et al. (2019)
5 more

1 mention without citation

The designability of protein switches by chemical rescue of structure: mechanisms of inactivation and reactivation.
Xia et al. (2013)