› 4bv4

X-ray diffraction
2.35Å resolution

Structure and allostery in Toll-Spatzle recognition

Released:
DOI: 10.2210/pdb4bv4/pdb
PDB entry 4bv4 coloured by chain, front view.
PDB entry 4bv4 coloured by chain, side view.
PDB entry 4bv4 coloured by chain, top view.
Source organisms:
Primary publication:
Cytokine Spatzle binds to the Drosophila immunoreceptor Toll with a neurotrophin-like specificity and couples receptor activation.
Lewis M, Arnot CJ, Beeston H, McCoy A, Ashcroft AE, Gay NJ, Gangloff M
Proc Natl Acad Sci U S A 110 20461-6 (2013)
PMID: 24282309

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140444 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein spaetzle C-106 Chains: L, M
Molecule details ›
Chains: L, M
Length: 107 amino acids
Theoretical weight: 11.99 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P48607 (Residues: 221-326; Coverage: 35%)
Gene names: CG6134, spz
Sequence domains: Spaetzle
Structure domains: Cystine-knot cytokines
LRRNT domain-containing protein; Protein toll Chain: R
Molecule details ›
Chain: R
Length: 446 amino acids
Theoretical weight: 50.75 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08953 (Residues: 28-397; Coverage: 35%)
  • Canonical: Q4G1L2 (Residues: 133-201; Coverage: 28%)
Gene names: CG5490, Tl, VLRB
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 198.31Å b: 57.11Å c: 70.5Å
α: 90° β: 97.76° γ: 90°
R-values:
R R work R free
0.197 0.195 0.227
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Assembly and localization of Toll-like receptor signalling complexes.
Gay et al. (2014)
6 more

4 mentions without citation

Cytokine Spatzle binds to the Drosophila immunoreceptor Toll with a neurotrophin-like specificity and couples receptor activation.
Lewis et al. (2013)
3 more