› 3sdj

X-ray diffraction
3.65Å resolution

Structure of RNase-inactive point mutant of oligomeric kinase/RNase Ire1

Released:
DOI: 10.2210/pdb3sdj/pdb
PDB entry 3sdj coloured by chain, front view.
PDB entry 3sdj coloured by chain, side view.
PDB entry 3sdj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural and functional basis for RNA cleavage by Ire1.
Korennykh AV, Korostelev AA, Egea PF, Finer-Moore J, Stroud RM, Zhang C, Shokat KM, Walter P
OpenAccess logo BMC Biol 9 47 (2011)
PMID: 21729333

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetradecamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase/endoribonuclease IRE1 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 448 amino acids
Theoretical weight: 51.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32361 (Residues: 641-1115; Coverage: 41%)
Gene names: ERN1, IRE1, YHR079C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand APJ 14 x APJ
2 modified residues:
Ligand SEP 28 x SEP
Ligand TPO 14 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 158.79Å b: 163.45Å c: 298.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.287
Expression system: Escherichia coli

Quick links

Citations

8 review citations

ER-stress in Alzheimer's disease: turning the scale?
Endres et al. (2013)
7 more

4 mentions without citation

Divergent allosteric control of the IRE1α endoribonuclease using kinase inhibitors.
Wang et al. (2012)
3 more