› 3p4b

X-ray diffraction
1.45Å resolution

Alternatingly modified 2'Fluoro RNA octamer f/rA2U2-P3

Released:
DOI: 10.2210/pdb3p4b/pdb
PDB entry 3p4b coloured by chain, front view.
PDB entry 3p4b coloured by chain, side view.
PDB entry 3p4b coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.
Pallan PS, Greene EM, Jicman PA, Pandey RK, Manoharan M, Rozners E, Egli M
Nucleic Acids Res 39 3482-95 (2011)
PMID: 21183463

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196715 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
5'-R(*(CFZ)P*GP*(AF2)P*AP*(UFT)P*UP*(CFZ)P*G)-3' Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 8 nucleotides
Theoretical weight: 2.53 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand BA 2 x BA
3 modified residues:
Ligand CFZ 12 x CFZ
Ligand AF2 6 x AF2
Ligand UFT 6 x UFT

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3
Unit cell:
a: 43.383Å b: 43.383Å c: 60.987Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.251
Expression system: Not provided

Quick links

Citations

23 review citations

Progress in microRNA delivery.
Zhang et al. (2013)
22 more

1 mention without citation

Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.
Pallan et al. (2011)