› 3kdz

X-ray diffraction
2.2Å resolution

X-ray crystal structure of a tyrosine aminomutase mutant construct with bound ligand

Released:
DOI: 10.2210/pdb3kdz/pdb
PDB entry 3kdz coloured by chain, front view.
PDB entry 3kdz coloured by chain, side view.
PDB entry 3kdz coloured by chain, top view.
Source organism: Streptomyces globisporus
Primary publication:
Probing the active site of MIO-dependent aminomutases, key catalysts in the biosynthesis of beta-amino acids incorporated in secondary metabolites.
Cooke HA, Bruner SD
Biopolymers 93 802-10 (2010)
PMID: 20577998

Function and Biology Details

Reactions catalysed: 
L-tyrosine = trans-p-hydroxycinnamate + ammonia
L-tyrosine = 3-amino-3-(4-hydroxyphenyl)propanoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-184536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MIO-dependent tyrosine 2,3-aminomutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 537 amino acids
Theoretical weight: 58.15 KDa
Source organism: Streptomyces globisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GMG0 (Residues: 1-539; Coverage: 100%)
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 2 x TYR
1 modified residue:
Ligand MDO 2 x MDO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 92.722Å b: 145.941Å c: 74.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.205 0.249
Expression system: Escherichia coli

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Citations

1 review citation

Enzymatic asymmetric synthesis of chiral amino acids.
Xue et al. (2018)
8 other articles

1 mention without citation

Probing the active site of MIO-dependent aminomutases, key catalysts in the biosynthesis of beta-amino acids incorporated in secondary metabolites.
Cooke et al. (2010)