› 3ecs

X-ray diffraction
2.65Å resolution

Crystal structure of human eIF2B alpha

Released:
DOI: 10.2210/pdb3ecs/pdb
PDB entry 3ecs coloured by chain, front view.
PDB entry 3ecs coloured by chain, side view.
PDB entry 3ecs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the alpha subunit of human translation initiation factor 2B.
Hiyama TB, Ito T, Imataka H, Yokoyama S
J Mol Biol 392 937-51 (2009)
PMID: 19631657

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor eIF2B subunit alpha Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 315 amino acids
Theoretical weight: 35.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 10 x SO4
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 70.885Å b: 156.394Å c: 138.441Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.214 0.214 0.273
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae.
Dever et al. (2016)
2 more

8 mentions without citation

eIF2B is a decameric guanine nucleotide exchange factor with a γ2ε2 tetrameric core.
Gordiyenko et al. (2014)
7 more