› 3dim

X-ray diffraction
2.9Å resolution

Crystallization of the Thermotoga maritima lysine riboswitch bound to lysine, Cs+ Soak

Released:
DOI: 10.2210/pdb3dim/pdb
PDB entry 3dim coloured by chain, front view.
PDB entry 3dim coloured by chain, side view.
PDB entry 3dim coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural insights into amino acid binding and gene control by a lysine riboswitch.
Serganov A, Huang L, Patel DJ
Nature 455 1263-7 (2008)
PMID: 18784651

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195853 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (174-MER) Chain: A
Molecule details ›
Chain: A
Length: 174 nucleotides
Theoretical weight: 56.64 KDa
Source organism: Thermotoga maritima
Expression system: Not provided
Sequence domains: Lysine riboswitch

Ligands and Environments

5 bound ligands:
Ligand CS 2 x CS
Ligand NA 15 x NA
Ligand LYS 1 x LYS
Ligand 1PE 1 x 1PE
Ligand IPA 1 x IPA
1 modified residue:
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 54.249Å b: 79.043Å c: 141.978Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.248
Expression system: Not provided

Quick links

Citations

49 review citations

A decade of riboswitches.
Serganov et al. (2013)
48 more

5 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
4 more