› 3sze

X-ray diffraction
2.5Å resolution

Crystal structure of the passenger domain of the E. coli autotransporter EspP

Released:
DOI: 10.2210/pdb3sze/pdb
PDB entry 3sze coloured by chain, front view.
PDB entry 3sze coloured by chain, side view.
PDB entry 3sze coloured by chain, top view.
Source organism: Escherichia coli O157:H7 str. EDL933
Primary publication:
Crystal structure of the passenger domain of the Escherichia coli autotransporter EspP.
Khan S, Mian HS, Sandercock LE, Chirgadze NY, Pai EF
J Mol Biol 413 985-1000 (2011)
PMID: 21964244

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Secreted autotransporter protein EspP Chain: A
Molecule details ›
Chain: A
Length: 968 amino acids
Theoretical weight: 105.54 KDa
Source organism: Escherichia coli O157:H7 str. EDL933
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7BSW5 (Residues: 56-1023; Coverage: 78%)
Gene names: ECO57PM78, L7020, espP
Sequence domains: Immunoglobulin A1 protease
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P43212
Unit cell:
a: 88.34Å b: 88.34Å c: 311.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis.
Leyton et al. (2012)
7 more

12 mentions without citation

Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity, and role in virulence.
Ruiz-Perez et al. (2014)
11 more