› 3spt

X-ray diffraction
2.33Å resolution

Crystal structure of GlmU from Mycobacterium tuberculosis in complex with ACETYL COENZYME A and URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE

Released:
DOI: 10.2210/pdb3spt/pdb
PDB entry 3spt coloured by chain, front view.
PDB entry 3spt coloured by chain, side view.
PDB entry 3spt coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Jagtap PA, Prakash B

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein GlmU Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 52.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMN3 (Residues: 1-495; Coverage: 100%)
Gene names: Rv1018c, glmU
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
2 bound ligands:
Ligand MG 3 x MG
Ligand UD1 1 x UD1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: R32
Unit cell:
a: 110.194Å b: 110.194Å c: 361.295Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.224
Expression system: Escherichia coli

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