› 2vzw

X-ray diffraction
2.3Å resolution

X-ray structure of the heme-bound GAF domain of sensory histidine kinase DosT of Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2vzw/pdb
PDB entry 2vzw coloured by chain, front view.
PDB entry 2vzw coloured by chain, side view.
PDB entry 2vzw coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
2.3 A X-ray structure of the heme-bound GAF domain of sensory histidine kinase DosT of Mycobacterium tuberculosis.
Podust LM, Ioanoviciu A, Ortiz de Montellano PR
Biochemistry 47 12523-31 (2008)
PMID: 18980385

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxygen sensor histidine kinase response regulator DosT Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.36 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WGK1 (Residues: 61-208; Coverage: 26%)
Gene names: Rv2027c, dosT
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
2 bound ligands:
Ligand ACT 2 x ACT
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P43
Unit cell:
a: 87.924Å b: 87.924Å c: 66.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.236 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Sensor domains of two-component regulatory systems.
Cheung et al. (2010)
12 more

7 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
6 more