› 2vyz

X-ray diffraction
1.8Å resolution

Mutant Ala55Phe of Cerebratulus lacteus mini-hemoglobin

Released:
DOI: 10.2210/pdb2vyz/pdb
PDB entry 2vyz coloured by chain, front view.
PDB entry 2vyz coloured by chain, side view.
PDB entry 2vyz coloured by chain, top view.
Source organism: Cerebratulus lacteus
Primary publication:
The apolar channel in Cerebratulus lacteus hemoglobin is the route for O2 entry and exit.
Salter MD, Nienhaus K, Nienhaus GU, Dewilde S, Moens L, Pesce A, Nardini M, Bolognesi M, Olson JS
J Biol Chem 283 35689-702 (2008)
PMID: 18840607

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neural hemoglobin Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 11.62 KDa
Source organism: Cerebratulus lacteus
Expression system: Escherichia coli
UniProt:
  • Canonical: O76242 (Residues: 1-110; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

5 bound ligands:
Ligand HEM 1 x HEM
Ligand OXY 1 x OXY
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 42.88Å b: 43.352Å c: 59.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.188 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A new paradigm for gaseous ligand selectivity of hemoproteins highlighted by soluble guanylate cyclase.
Wu et al. (2021)
1 more

2 mentions without citation

Determination of ligand pathways in globins: apolar tunnels versus polar gates.
Salter et al. (2012)
1 more